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HPTLC Application LabHPTLC Application Lab

HPTLC Application Lab

What is HPTLC

Chromatography is a laboratory method for analysis of chemicals to determine purity, impurities, similarity, identification etc. Chromatography today is performed with the help of different instruments, materials, techniques, as would be required to analyse hundreds of thousands of chemicals. The prominent chromatography methods are High Performance Liquid Chromatography (HPLC), High Performance Thin Layer Chromatography (HPTLC), Gas Chromatography (GC) etc.

Suggested reading:

High Performance Thin – Layer Chromatography (HPTLC)
Dr. M. M. Srivastava
 

High Performance Thin Layer Chromatography (HPTLC) is the modern instrumental way of performing the classical Thin Layer Chromatography method of analysis. HPTLC requires the use of instruments, precoated thin layers and experiments are designed for quantification. HPTLC complies with all modern GLP and GMP requirements.

Main advantages of HPTLC are that:

  • The "Chromatogram" is visible to the eye
  • Contamination risk – free analysis
  • Very simple to use
  • Very low running and maintenance cost
  • Thousands of ready references
  • Extremely flexible detection
  • Hyphenated to Mass Spectrometry

Suggested reading:

Name of Book
Author
Quantitative Analysis of Pharmaceutical Formulations
Dr. P. D. Sethi
High – Performance Thin – Layer Chromatography in food analysis
Dr. P. K. Jaiswal
 
 

Some results from HPTLC

Image at 254nm Image at 366nm 3-D PLOT Calibration Curve
Image at 254nm Image at 366nm 3-D PLOT Calibration Curve
UV Spectra Mass Spectra Bioluminizer Bioautography
UV Spectra Mass Spectra Bioluminizer Bioautography